Selected Publications

Waheed, A.A., Ablan, S.D., Soheilian, F., Nagashima, K., Ono, A., Schaffner, C.P., and Freed, E.O.  (2008)  Inhibition of human immunodeficiency virus type 1 assembly and release by the cholesterol-binding compound amphotericin B methyl ester: Evidence for Vpu dependence.  J. Virol., in press.

Saad, J.S., Ablan, S.D., Ghanam, R.H., Kim, A., Andrews, K., Nagashima, K., Freed, E.O., and Summers, M.F.  (2008)  Structure of the myristylated HIV-2 MA protein and the role of PI(4,5)P₂ in membrane targeting.  J. Mol Biol., in press.

Liu, F., Stephen, A.G., Waheed, A.A., Aman, M.J., Freed, E.O., Fisher, R.J., and Burke, T.R., Jr.  (2008)  SAR by oxime-containing peptide libraries: Application to Tsg101 ligand optimization as an approach towards new anti-HIV-1 therapeutics.  ChemBioChem, in press.

Waheed, A.A., Ono, A., and Freed, E.O.  (2008)  Methods for the study of HIV-1 assembly.  In HIV Protocols, 2nd Ed., Humana Press, in press.

Adamson, C.S., and Freed, E.O.  (2008)  Recent progress in antiretrovirals – lessons from resistance.  Drug Discov. Today 13: 424-432.
[Abstract]     [Full-text PDF article - 627KB)]

Joshi, A., Garg, H., Nagashima, K., Bonifacino, J.S., and Freed, E.O.  (2008)  GGA and Arf proteins modulate retrovirus assembly and release.  Mol. Cell 30: 227-238.
[Abstract]     [Full-text PDF article - 1606KB]

Zhang, H., Zhao, Q., Bhattacharya, S., Waheed, A.A., Tong, X., Hong, A., Heck, S., Curreli, F., Goger, M., Cowburn, D., Freed, E.O., and Debnath, A.K.  (2008)  A cell-penetrating helical peptide as a potential HIV-1 inhibitor.  J. Mol. Biol. 378: 565-580.
[Abstract]     [Full-text PDF article - 1062KB]

Gousset, K., Ablan, S.D., Coren, L.V., Ono, A., Soheilian, F., Nagashima, K., Ott, D.E., and Freed, E.O.  (2008)  Real-time visualization of HIV-1 Gag trafficking in infected macrophages.  PLoS Pathog. 4: e1000015.
[Abstract]     [Full-text PDF article - 667KB]

Viard, M., Ablan, S.D., Zhou, M., Veenstra, T.D., Freed, E.O., Raviv, Y., and Blumenthal, R.  (2008)  Photoinduced reactivity of the HIV-1 envelope glycoprotein with a membrane-embedded probe reveals insertion of portions of the HIV-1 gp41 cytoplasmic tail into the viral membrane.  Biochemistry 47: 1977-1983.
[Abstract]     [Full-text PDF article - 227KB]

Luttge, B.G., Shehu-Xhilaga, M., Demirov, D.G., Adamson, C.S., Soheilian, F., Nagashima, K., Stephen, A.G., Fisher, R.J., and Freed, E.O..  (2008)  Molecular characterization of feline immunodeficiency virus budding.  J. Virol. 82: 2106-2109.
[Abstract]     [Full-text PDF article - 2408KB]

Fujii, K., Hurley, J.H., and Freed, E.O.  (2007)  Beyond Tsg101: The role of Alix in 'ESCRTing' HIV-1.  Nat. Rev. Microbiol. 5: 912-916.
[Abstract]     [Full-text PDF article - 2408KB]

Joshi, A., and Freed, E.O.  (2007)  HIV-1 Gag trafficking.  Future HIV Ther. 1: 427-438.
[Abstract]     [Full-text PDF article - 936KB]

Waheed, A.A., and Freed, E.O.  (2007)  Influenza virus not cRAFTy enough to dodge viperin.  Cell Host Microbe 2: 71-72.     [Abstract]     [Full-text PDF article - 229KB]

Adamson, C.S., and Freed, E.O.  (2007)  Human immunodeficiency virus type 1 assembly, release, and maturation.  In Jeang, K.-T. (ed.), HIV-1: Molecular Biogenesis and Pathogenesis: Viral Mechanisms, 2nd Ed., Advances in Pharmacology, Vol. 55, Elsevier Inc.  pp. 347-387.     [PubMed citation]

Garg, H., Joshi, A., Freed, E.O., and Blumenthal, R.  (2007)  Site-specific mutations in HIV-1 gp41 reveal a correlation between HIV-1-mediated bystander apoptosis and fusion/hemifusion. J. Biol. Chem. 282: 16899-16906.
[Abstract]     [Full-text PDF article - 657KB]

Waheed, A.A., Ablan, S.D., Roser, J.D., Sowder, R.C., Schaffner, C.P., Chertova, E., and Freed, E.O.  (2007)  HIV-1 escape from the entry-inhibiting effects of a cholesterol-binding compound via cleavage of gp41 by the viral protease.  Proc. Natl. Acad. Sci. USA 104: 8467-8471.     [Abstract]     [Full-text PDF article - 675KB]

Lee, S., Joshi, A., Nagashima, K., Freed, E.O., and Hurley, J.H.  (2007)  Structural basis for viral late-domain binding to Alix.  Nat. Struct. Mol. Biol. 14: 194-199.
[Abstract]     [Full-text PDF article - 631KB]

Ono, A., Waheed, A.A., and Freed, E.O.  (2007)  Depletion of cellular cholesterol inhibits membrane binding and higher-order multimerization of human immunodeficiency virus type 1 Gag.  Virology 360: 27-35.     [Abstract]     [Full-text PDF article - 490KB]

Freed, E.O., and Martin, M.A.  (2007)  HIVs and their replication.  Chapter 57. In Knipe, D.M., and Howley, P.M. (eds.), Fields Virology, 5th Ed., Lippincott, Williams, and Wilkins: Philadelphia.  pp. 2107-2186.

Munshi, U.M., Kim, J., Nagashima, K., Hurley, J.H., and Freed, E.O.  (2007)  An Alix fragment potently inhibits HIV-1 budding: Characterization of binding to retroviral YPXL late domains.  J. Biol. Chem. 282: 3847-3855.
[Abstract]     [Full-text PDF article - 492KB]

Saad, J.S., Loeliger, E., Luncsford, P., Liriano, M., Tai, J., Kim, A., Miller, J., Joshi, A., Freed, E.O., and Summers, M.F.  (2007)  Point mutations in the HIV-1 matrix protein turn off the myristyl switch.  J. Mol. Biol. 366: 574-585.
[Abstract]     [Full-text PDF article - 1814KB]

Tang, S., Ablan, S., Dueck, M., Ayala-López, W., Soto, B., Caplan, M., Nagashima, K., Hewlett, I.K., Freed, E.O., and Levin, J.G.  (2007)  A second-site suppressor significantly improves the defective phenotype imposed by mutation of an aromatic residue in the N-terminal domain of the HIV-1 capsid protein.  Virology 359: 105-115.
[Abstract]     [Full-text PDF article - 1613KB]

Freed, E.O., and Mouland, A.O.  (2006)  Meeting Review: The cell biology of HIV-1 and other retroviruses.  Retrovirology 3: 77-87.
[Abstract]      [Full-text PDF article - 444KB]

Li, F., Zoumplis, D., Matallana, C., Kilgore, N.R., Reddick, M., Yunus, A.S., Adamson, C.S., Salzwedel, K., Martin, D.E., Allaway, G.P., Freed, E.O., and Wild, C.T.  (2006)  Determinants of activity of the HIV-1 maturation inhibitor PA-457.  Virology 356: 217-224.     [Abstract]     [Full-text PDF article - 571KB]

Adamson, C.S., Ablan, S.D., Boeras, I., Goila-Gaur, R., Soheilian, F., Nagashima, K., Li, F., Salzwedel, K., Sakalian, M., Wild, C.T., and Freed, E.O.  (2006)  In vitro resistance to the human immunodeficiency virus type 1 maturation inhibitor PA-457 (bevirimat).  J. Virol. 80: 10957-10971.     [Abstract]     [Full-text PDF article - 1326KB]

Liu, F., Stephen, A.G., Adamson, C.S., Gousset, K., Aman, M.J., Freed, E.O., Fisher, R.J., and Burke, T.R., Jr.  (2006)  Hydrazone- and hydrazide-containing N-substituted glycines as peptoid surrogates for expedited library synthesis: Application to the preparation of Tsg101-directed HIV-1 budding antagonists.  Org. Lett. 8: 5165-5168.
[Abstract]     [Full-text PDF article - 127KB]

Waheed, A.A., Ablan, S.D., Mankowski, M.K., Cummins, J.E., Ptak, R.G., Schaffner, C.P., and Freed, E.O.  (2006)  Inhibition of HIV-1 replication by amphotericin B methyl ester: Selection for resistant variants.  J. Biol. Chem. 281: 28699-28711.
[Abstract]     [Full-text PDF article - 779KB]

Joshi, A., Nagashima, K., and Freed, E.O.  (2006)  Mutation of dileucine-like motifs in the human immunodeficiency virus type 1 capsid disrupts virus assembly, Gag-Gag interactions, Gag-membrane binding, and virion maturation.  J. Virol. 80: 7939-7951.
[Abstract]     [Full-text PDF article - 725KB]

Freed, E.O.  (2006)  HIV-1 Gag: Flipped out for PI(4,5)P₂.  Proc. Natl. Acad. Sci. USA 103: 11101-11102.     [PubMed citation]      [Full-text PDF article - 224KB]

Davis, M.R., Jiang, J., Zhou, J., Freed, E.O., and Aiken, C.  (2006)  A mutation in the human immunodeficiency virus type 1 Gag protein destabilizes the interaction of the envelope protein subunits gp120 and gp41.  J. Virol. 80: 2405-2417.
[Abstract]     [Full-text PDF article - 591KB]

Fu, W., Dang, Q., Nagashima, K., Freed, E.O., Pathak, V.K., and Hu, W.-S.  (2006)  Effects of Gag mutation and processing on retroviral dimeric RNA maturation.  J. Virol. 80: 1242-1249.     [Abstract]     [Full-text PDF article - 367KB]

Ono, A., Waheed, A.A., Joshi, A., and Freed, E.O.  (2005)  Association of human immunodeficiency virus type 1 Gag with membrane does not require highly basic sequences in the nucleocapsid: Use of a novel Gag multimerization assay.  J. Virol. 79: 14131-14140.     [Abstract]     [Full-text PDF article - 337KB]

Ono, A., and Freed, E.O.  (2005)  Role of lipid rafts in virus replication.  Adv. Virus Res. 64: 311-358.     [PubMed citation]

Sklar, P.A., Masur, H., Grubb, J.R., Voell, J., Witek, J., Ono, A., Freed, E.O., and Maldarelli, F.  (2005)  Pravastin does not have a consistent antiviral effect in chronically HIV-infected individuals on antiretroviral therapy.  AIDS 19: 1109-1111.
[PubMed citation]     [Full-text PDF article - 75KB]

Demirov, D.G., and Freed, E.O.  (2004)  Retrovirus budding.  In Mechanisms of Enveloped Virus Release (E.O. Freed, ed.), Virus Research, Vol. 106, pp. 87-102.
[Abstract]     [Full-text PDF article - 441KB]

Freed, E.O.  (2004)  Preface.  In Mechanisms of Enveloped Virus Release (E.O. Freed, ed.), Virus Research, Vol. 106, pp. 85-86.
[PubMed citation]     [Full-text PDF article - 31KB]

Ono, A., Ablan, S.D., Lockett, S.J., Nagashima, K., and Freed, E.O.  (2004)  Phosphatidylinositol (4,5) bisphosphate regulates HIV-1 Gag targeting to the plasma membrane.  Proc. Natl. Acad. Sci. USA 101: 14889-14894.
[Abstract]     [Full-text PDF article - 886KB]

Freed, E.O., and Ross, S.R.  (2004)  Retroviruses 2004: Review of the 2004 Cold Spring Harbor Retroviruses conference.  Retrovirology 1: 25 (E-pub).
[Abstract]     [Full-text PDF article - 227KB]

Svarovskaia, E.S., Xu, H., Mbisa, J.L., Barr, R., Gorelick, R.J., Ono, A., Freed, E.O., Hu, W.-S., and Pathak, V.K.  (2004)  Human apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) is incorporated into HIV-1 virions through interactions with viral and nonviral RNAs.  J. Biol. Chem. 279: 35822-35828.
[Abstract]     [Full-text PDF article - 356KB]

Freed, E.O.  (2004)  HIV-1 and the host cell: An intimate association.  Trends Microbiol. 12: 170-177.     [Abstract]     [Full-text PDF article - 237KB]

Ono, A., and Freed, E.O.  (2004)  Cell-type-dependent targeting of human immunodeficiency virus type 1 assembly to the plasma membrane and the multivesicular body.  J. Virol. 78: 1552-1563.
[Abstract]     [Full-text PDF article - 4759KB]

Murakami, T., Ablan, S., Freed, E.O., and Tanaka, Y.  (2004)  Regulation of human immunodeficiency virus type 1 Env-mediated membrane fusion by viral protease activity.  J. Virol. 78: 1026-1031.
[Abstract]     [Full-text PDF article - 266KB]

Shehu-Xhilaga, M., Ablan, S., Demirov, D.G., Chen, C., Montelaro, R.C., and Freed, E.O.  (2004)  Late domain-dependent inhibition of equine infectious anemia virus budding.  J. Virol. 78: 724-732.     [Abstract]     [Full-text PDF article - 928KB]

Tang, S., Murakami, T., Cheng, N., Stephen, A.C., Freed, E.O., and Levin, J.G.  (2003)  Human immunodeficiency virus type 1 N-terminal capsid mutants containing cores with abnormally high levels of capsid protein and virtually no reverse transcriptase.  J. Virol. 77: 12592-12602.     [Abstract]     [Full-text PDF article - 1064KB]

Li, F., Goila-Gaur, R., Salzwedel, K., Kilgore, N.R., Reddick, M., Matallana, C., Castillo, A., Zoumplis, D., Martin, D.E., Orenstein, J.M., Allaway, G.P., Freed, E.O., and Wild, C.T.  (2003)  PA-457: A potent HIV inhibitor that disrupts core condensation by targeting a late step in Gag processing.  Proc. Natl. Acad. Sci. USA 100: 13555-13560.
[Abstract]     [Full-text PDF article - 383KB]

Goila-Gaur, R., Demirov, D.G., Orenstein, J.M, Ono, A., and Freed, E.O.  (2003)  Defects in human immunodeficiency virus budding and endosomal sorting induced by TSG101 overexpression.  J. Virol. 77: 6507-6519.
[PubMed citation]     [Full-text PDF article - 3180KB]

Freed, E.O.  (2003)  The HIV-TSG101 interface: Recent advances in a budding field.  Trends Microbiol. 11: 56-59.     [Abstract]

Freed, E.O.  (2002)  Viral late domains.  J. Virol. 76: 4679-4687.
[PubMed citation]     [Full-text PDF article - 726KB]

Freed, E.O.  (2002)  Rafting with Ebola.  Science 296: 279.
[PubMed citation]     [Full-text PDF article - 54KB]

Demirov, D.G., Orenstein, J.M., and Freed, E.O.  (2002)  The late domain of human immunodeficiency virus type 1 p6 promotes virus release in a cell type-dependent manner.  J. Virol. 76: 105-117.     [Abstract]     [Full-text PDF article - 1800KB]

Demirov, D.G., Ono, A., Orenstein, J.M., and Freed, E.O.  (2002)  Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain function.  Proc. Natl. Acad. Sci. USA 99: 955-960.
[Abstract]     [Full-text PDF article - 400KB]

Freed, E.O., and Martin, M.A.  (2001)  HIVs and their replication.  In Knipe, D.M., and Howley, P.M. (eds.), Fields Virology, Chapter 59, 4th Ed., Lippincott, Williams, and Wilkins: Philadelphia, pp. 1971-2041.

Ono, A., and Freed, E.O.  (2001)  Plasma membrane rafts play a critical role in HIV-1 assembly and release.  Proc. Natl. Acad. Sci. USA 98: 13925-13930.
[Abstract]     [Full-text PDF article - 353KB]

Ono, A., Demirov, D., and Freed, E.O.  (2000)  Relationship between human immunodeficiency virus type 1 Gag multimerization and membrane binding.  J. Virol. 74: 5142-5150.     [Abstract]     [Full-text PDF article - 1524KB]

Murakami, T., and Freed, E.O.  (2000)  Genetic evidence for an interaction between human immunodeficiency virus type 1 matrix and alpha-helix 2 of the gp41 cytoplasmic tail.  J. Virol. 74: 3548-3554.     [Abstract]     [Full-text PDF article - 530KB]

Ono, A., Orenstein, J.M., and Freed, E.O.  (2000)  Role of the Gag matrix domain in targeting human immunodeficiency virus type 1 assembly.  J. Virol. 74: 2855-2866.
[Abstract]     [Full-text PDF article - 4484KB]

Murakami, T., and Freed, E.O.  (2000)  The long cytoplasmic tail of gp41 is required in a cell type-dependent manner for HIV-1 envelope glycoprotein incorporation into virions.  Proc. Natl. Acad. Sci. USA 97: 343-348.
[Abstract]     [Full-text PDF article - 225KB]

Last modified: 11 July 2008